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Title   흰쥐에서 성호르몬이 태반형 Glutathione S - Transferase 양성 증식성 간결절 유도에 미치는 영향 ( Effects of Sex Hormones on the Induction of Glutathione S - Transferase - P Positive Hyperplastic Hepatic Nodules in Rats )
Publicationinfo   1991 Jan; 023(04): 708-723.
Key_word   Hepatocellular carcinoma, Sex hormone, GST-P, Promotion
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Abstract   To elucidste the mechanism of male preponderance phenomenon in human hepatocellular carcinoma, chemical hepatocarcinogenic procedure was applied to Sprague-Dawley rats after Solt-Farber model which allowed early histologic, biochemical detection of altered hepatic lesions. At different times prior ta during the procedure, some groups of the rats underwent gonadectomy in order to evaluate effects of sex hormones on the induction of preneoplastic lesions, i.e., glutathione S-transferase-P(GST-P) positive hyperplastic hepatic nodules. Sex hormone such as testosterone or estradiol was replaced in another group of orchiectomized or oophorectomized rats to ascertain the relationship between gonadectomy and defect in sex hormone. The results were analysed by immunohistochemical study and biochemical method using anti-GST-P antibody, and summarized as follows: 1) Male rats without orchiectomy showed significantly larger area of GST-P positive foci than female rats without oophorectomy (p<0.05). 2) Orchiectomy aiways decreased remarkably the area of GST-P positive foci than in the non-orchiectomized(0.05). 3) Earlier orchiectomy resulted in more decreased area of GST-P positive foci. 4) Replacement of testosterone ta the orchiectamized rats recovered the previously de- creased area of GST-P positive foci(p<0.05). 5) Oophorectomy significantly increased the area of GST-P positive foci than in the non- oophorectomized(p < 0.05). 6) Replacement of estradiol to the oophorectomized rats reduced the previously increased area of GST-P positive foci again(p<0.05). 7) Western blot analysis showed comparable results to immunohistochemical study. These results suggest that during hepatocarcinogenesis male sex hormone augments the ap- pearance of preneoplastic lesions and female sex hormone suppresses it and that male sex hormone has effects on promotion rather than initiation of carcinogenesis. These may provide one of the experimental backgrounds which explain the mechanism of male preponderance in human hepatocarcinogenesis.
저 자   이상전(Sang Jeon Lee),김수태(Soo Tae Kim),박상철(Sang Chul Park),송계용(Kye Yong Song)